English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

This study assesses a novel bile solubility test and MALDI-TOF for the differentiation of  Streptococcus pneumoniae  from other mitis group streptococci, including differentiation of  S. pneumoniae  from  Streptococcus pseudopneumoniae . Eighty-four species verified mitis group isolates were subjected to our bile solubility test (which measures and calculates the differences of absorbance in the test tube containing 10% sodium deoxycholate versus a blank control tube, after incubation for 10 minutes at 36 °C using a spectrophotometer) and MALDI-TOF MS (both the standard result output and by visual spectra evaluation). Applying a calculated optimal cut-off absorbance-value of 2.1, differentiated  S. pneumoniae  from all but one other mitis group streptococci (one  S. mitis  isolate generated an OD-value above 2.1). MALDI-TOF score value identification identified correctly 46  S. pneumoniae  and 4  S. pseudopneumoniae  but misidentified 16 other mitis group strains. Visual spectra evaluation correctly identified all  S. pneumoniae  and  S. pseudopneumoniae  strains but misidentified 13 other mitis group strains. The bile solubility test based on spectrophotometric reading described in this study can differentiate  S. pneumoniae  from other  Streptococcus  species. Combining the bile solubility test and the MALDI-TOF spectra results provide a correct identification of all  S. pneumoniae  and  S. pseudopneumoniae  isolates.

Assessment of a novel bile solubility test and MALDI-TOF for the differentiation of Streptococcus pneumoniae from other mitis group streptococci