English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

During the course of infection, pathogenic mycobacteria including  Mycobacterium tuberculosis  ( M .  tb ) encounter host environments of variable oxygen tension, ranging from the hypoxic center of granulomas to the most oxygenated region in the lung cavities. Mycobacterial responses to changes of oxygen tension are critically related to infection outcomes, such as latency and reactivation. WhiB4 is an iron-sulfur containing transcription factor that is highly sensitive to oxygen exposure. In this study, we found that WhiB4 of  Mycobacterium marinum  ( M .  marinum ), a pathogenic mycobacterial species that is closely related to  M .  tb , is required for its virulence.  M .  marinum  Δ whiB4  exhibited defective intracellular replication in macrophages and diminished virulence in zebrafish. Histology analysis revealed that the host had successfully controlled Δ whiB4  bacteria, forming well-organized granulomas. RNA-seq analysis identified a large number of  pe / ppe  genes that were regulated by WhiB4, which provides an explanation for the essential role of WhiB4 in  M .  marinum  virulence. Several antioxidant enzymes were also upregulated in Δ whiB4 , supporting its role in modulation of oxidative stress response. Taken together, we have provided new insight into and proposed a model to explain the physiological role of WhiB4.

WhiB4 Regulates the PE/PPE Gene Family and is Essential for Virulence of Mycobacterium marinum