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A global insight into the roles of multiple P-type calcium ATPase (CA) pumps in sustaining the life of a filamentous fungal pathogen is lacking. Here we elucidated the functions of five CA pumps (Eca1, Spf1 and PmcA/B/C) following previous characterization of Pmr1 in  Beauveria bassiana , a fungal insect pathogen. The fungal CA pumps interacted at transcriptional level, at which singular deletions of five CA genes depressed  eca1  expression by 76–98% and deletion of  spf1  resulted in drastic upregulation of four CA genes by 36–50-fold. Intracellular Ca 2+  concentration increased differentially in most deletion mutants exposed to the stresses of Ca 2+ , EDTA chelator, and/or endoplasmic reticulum and calcineurin inhibitors, accompanied with their changed sensitivities to not only the mentioned agents but also Fe 2+ , Cu 2+  and Zn 2+ . Liquid culture acidification was delayed in the Δ spf1 , Δ pmr1  and Δ pmcA  mutants, coinciding well with altered levels of their extracellular lactic and oxalic acids. Moreover, all deletion mutants showed differential defects in conidial germination, vegetative growth, conidiation capacity, antioxidant activity, cell wall integrity, conidial UV-B resistance and/or virulence. Our results provide the first global insight into differential roles for six CA pumps in sustaining intracellular Ca 2+  level, asexual cycle and environmental fitness of  B .  bassiana .

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Differential Roles for Six P-Type Calcium ATPases in Sustaining Intracellular Ca2+ Homeostasis, Asexual Cycle and Environmental Fitness of Beauveria bassiana