English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Commercial malaria rapid diagnostic tests (RDTs) detect  P. falciparum  histidine rich protein 2 ( Pf HRP2) and cross react with  Pf HRP3, a structural homologue. Here, we analysed natural variations in  Pf HRP2 and  Pf HRP3 sequences from Indian isolates and correlated these variations with RDT reactivity. A total 1392  P. falciparum  positive samples collected from eight endemic states were PCR amplified for  Pfhrp2  and  Pfhrp3  genes and were sequenced. The deduced protein sequences were analysed for repeat variations and correlated with RDT reactivity. Out of 1392 PCR amplified samples, a single sample was  Pfhrp2  negative and two samples were  Pfhrp3  negative. Complete  Pfhrp 2 and  Pfhrp 3 sequences were obtained for 769 samples and 750 samples, respectively. A total of 16 distinct repeat motifs were observed for  Pfhrp2  and 11 for  Pfhrp3 , including some new repeat types. No correlation was found between variations in the size of  Pfhrp2  repeat types 2 and 7, nor between any combinations of repeat motifs, and performance of a commercial RDT at low parasite densities. The findings suggest that sequence diversity in  Pfhrp2  and  Pfhrp3  genes in Indian isolates is not likely to negatively influence performance of currently used  Pf HRP2 RDTs.

scientific reports

Sequence variation in Plasmodium falciparum Histidine Rich Proteins 2 and 3 in Indian isolates: Implications for Malaria Rapid Diagnostic Test Performance