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Larvae of most lepidopteran insect species are known to be voracious feeders and important agricultural pests throughout the world.  Achaea janata  larvae cause serious damage to  Ricinus communis  (Castor) in India resulting in significant economic losses. Microbial insecticides based on crystalline (Cry) toxins of  Bacillus thuringiensis  (Bt) have been effective against the pest. Excessive and indiscriminate use of Bt - based biopesticides could be counter-productive and allow susceptible larvae to eventually develop resistance. Further, lack of adequate genome and transcriptome information for the pest limit our ability to determine the molecular mechanisms of altered physiological responses in Bt-exposed susceptible and tolerant insect strains. In order to facilitate biological, biochemical and molecular research of the pest species that would enable more efficient biocontrol, we report the midgut  de novo  transcriptome assembly and clustering of susceptible Cry toxin-exposed and Cry toxin tolerant  Achaea janata  larvae with appropriate age-matched and starvation controls.

RNA-Seq analysis and de novo transcriptome assembly of Cry toxin susceptible and tolerant Achaea janata larvae