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In today's clinics, a cellular-resolution view of the cornea can be achievedonly with an in vivo confocal microscope (IVCM) in contact with the eye. Here,we present a common-path Full-field/Spectral-domain OCT microscope (FF/SD OCT),which, for the first time, enables cell-detail imaging of the entire ocularsurface (central and peripheral cornea, limbus, sclera, tear film) withoutcontact and in real time. The device, which has been successfully tested inhuman subjects, is now ready for direct implementation in clinical research.Real-time performance is achieved through rapid axial eye tracking andsimultaneous defocusing correction. Images, extracted from real-time videos,contain cells and nerves, which can be quantified over a millimetricfield-of-view, beyond the capability of IVCM and conventional OCT. In thelimbus, Palisades of Vogt, vessels and blood flow can be resolved with highcontrast without contrast agent injection. The fast imaging speed of 275frames/s (0.6 billion pixels/s) allowed direct monitoring of blood flowdynamics, enabling creation of high-resolution velocity maps for the firsttime. Tear flow velocity and evaporation time could be measured withoutfluorescein administration.

Real-time, non-contact, cellular imaging and angiography of human cornea and limbus with common-path Full-field/SDOCT