English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Grapevine downy mildew (DM) is a destructive oomycete disease of viticulture worldwide.  MrRPV1  is a typical TIR-NBS-LRR type DM disease resistance gene cloned from the wild North American grapevine species  Muscadinia rotundifolia . However, the molecular basis of resistance mediated by  MrRPV1  remains poorly understood. Downy mildew-susceptible  Vitis vinifera  cv. Shiraz was transformed with a genomic fragment containing  MrRPV1  to produce DM-resistant transgenic Shiraz lines. Comparative transcriptome analysis was used to compare the transcriptome profiles of the resistant and susceptible genotypes after DM infection. Transcriptome modulation during the response to  P. viticola  infection was more rapid, and more genes were induced in  MrRPV1 -transgenic Shiraz than in wild-type plants. In DM-infected  MrRPV1 -transgenic plants, activation of genes associated with Ca 2+  release and ROS production was the earliest transcriptional response. Functional analysis of differentially expressed genes revealed that key genes related to multiple phytohormone signaling pathways and secondary metabolism were highly induced during infection. Coexpression network and motif enrichment analysis showed that WRKY and MYB transcription factors strongly coexpress with stilbene synthase ( VvSTS ) genes during defense against  P. viticola  in  MrRPV1 -transgenic plants. Taken together, these findings indicate that multiple pathways play important roles in  MrRPV1- mediated resistance to downy mildew.

horticulture research

Transcriptional profiling reveals multiple defense responses in downy mildew-resistant transgenic grapevine expressing a TIR-NBS-LRR gene located at the MrRUN1/MrRPV1 locus