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In plants, alternative splicing (AS) is markedly induced in response to environmental stresses, but it is unclear why plants generate multiple transcripts under stress conditions. In this study, RNA-seq was performed to identify AS events in cucumber seedlings grown under different light intensities. We identified a novel transcript of the gibberellin (GA)-deactivating enzyme  Gibberellin 2-beta-dioxygenase  8 ( CsGA2ox8 ). Compared with canonical  CsGA2ox8.1 , the  CsGA2ox8.2  isoform presented intron retention between the second and third exons. Functional analysis proved that the transcript of  CsGA2ox8.1  but not  CsGA2ox8.2  played a role in the deactivation of bioactive GAs. Moreover, expression analysis demonstrated that both transcripts were upregulated by increased light intensity, but the expression level of  CsGA2ox8.1  increased slowly when the light intensity was >400 µmol·m −2 ·s −1  PPFD (photosynthetic photon flux density), while the  CsGA2ox8.2  transcript levels increased rapidly when the light intensity was >200 µmol·m −2 ·s −1  PPFD. Our findings provide evidence that plants might finely tune their GA levels by buffering against the normal transcripts of  CsGA2ox8  through AS.

horticulture research

Plant buffering against the high-light stress-induced accumulation of CsGA2ox8 transcripts via alternative splicing to finely tune gibberellin levels and maintain hypocotyl elongation