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In plants, the vesicle fusion process plays a vital role in pathogen defence. However, the importance of the vesicle fusion process in apple ring rot has not been studied. Here, we isolated and characterised the apple syntaxin gene  MdSYP121 . Silencing the  MdSYP121  gene in transgenic apple calli increased tolerance to  Botryosphaeria dothidea  infection; this increased tolerance was correlated with salicylic acid (SA) synthesis-related and signalling-related gene transcription. In contrast, overexpressing  MdSYP121  in apple calli resulted in the opposite phenotypes. In addition, the results of RNA sequencing (RNA-Seq) and quantitative real-time PCR (qRT-PCR) assays suggested that  MdSYP121  plays an important role in responses to oxidation–reduction reactions. Silencing  MdSYP121  in apple calli enhanced the expression levels of reactive oxygen species (ROS)-related genes and the activity of ROS-related enzymes. The enhanced defence response status in MdSYP121-RNAi lines suggests that syntaxins are involved in the defence response to  B. dothidea . More importantly, we showed that MdSYP121 forms a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex with MdSNAP33, and the complex may participate in regulating resistance to  B. dothidea . In conclusion, by regulating the interaction of SA pathway and oxidation–reduction process,  MdSYP121  can influence the pathogen infection process in apple.

Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121 in apple