English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Primary cilia are antenna-like subcellular structures to act as signaling platforms to regulate many cellular processes and embryonic development. m 1 A RNA modification plays key roles in RNA metabolism and gene expression; however, the physiological function of m 1 A modification remains largely unknown. Here we find that the m 1 A demethylase ALKBH3 significantly inhibits ciliogenesis in mammalian cells by its demethylation activity. Mechanistically, ALKBH3 removes m 1 A sites on mRNA of  Aurora A , a master suppressor of ciliogenesis. Depletion of ALKBH3 enhances  Aurora A  mRNA decay and inhibits its translation. Moreover,  alkbh3  morphants exhibit ciliary defects, including curved body, pericardial edema, abnormal otoliths, and dilation in pronephric ducts in zebrafish embryos, which are significantly rescued by wild-type  alkbh3 , but not by its catalytically inactive mutant. The ciliary defects caused by ALKBH3 depletion in both vertebrate cells and embryos are also significantly reversed by ectopic expression of  Aurora A  mRNA. Together, our data indicate that ALKBH3-dependent m 1 A demethylation has a crucial role in the regulation of  Aurora A  mRNA, which is essential for ciliogenesis and cilia-associated developmental events in vertebrates.

ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis