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The development of optical microscopy techniques has provided major insights into the machinery of life, from molecules to cells to tissues and organs. Fluorescent techniques have been particularly successful in this respect by elucidating biological pathways and helping us understand the complex relationships that underpin all living organisms. For example, the first use of fluorescence as an analytical tool in 1864 paved the way for the study of biological entities and interactions, but it was not until nearly 60 years later, in the 1930s, that fluorophores were first employed to perform biological investigations into specific tissue components and bacteria that do not autofluoresce. These advances led to the first labeling of an antibody with a visible label in 1941, which gave birth to the field of immunofluorescence and allowed us obtain unprecedented insight into antibody structure. Nevertheless, fluorescence techniques require the addition of labels, which complicate the procedure and may interfere with the very pathways that we are trying to understand. As a result, new methods have been developed that do not require the addition of fluorescent labels but use photonic resonances to enhance the weak interaction between electromagnetic fields and the biological objects of interest. Moreover, it is important to detect multiple parameters in parallel since information about the density, adhesion, or motility of a living body and properties such as the stiffness and impedance of biological objects all provide insights into our understanding of biological pathways. An important step toward this goal of multiparameter characterization has now been taken with the introduction of a resonant labelfree microscopy technique that offers insights into both the density of cells and their adhesion to a substrate. The technique exploits guided mode resonances in photonic crystals and is termed photonic resonator outcoupler microscopy (PROM). PROM can detect changes in both the phase and amplitude of the photonic resonance that is caused by the presence of a cell. The change in phase is related to the optical density, i.e., it indicates the presence of cellular matter, while the change in amplitude is related to the shape of the cell, which indicates adhesion to the surface. Related techniques include surface plasmon resonance microscopy (SPRM), photonic crystal enhanced microscopy (PCEM) and elastic resonator interference stress microscopy (ERSIM). SPRM and PCEM sense changes in the refractive index (n) within the first ~200 nm from the substrate and have demonstrated the capability of monitoring cell attachment and protein diffusion at the cell membrane level, while ERSIM detects adhesion and, more generally, forces that are exerted by the cell on the surface. Deeper, complementary biological information can be retrieved with PROM by examining the refractive index variations more carefully; PROM is able to disentangle changes in the refractive index from changes in scattering, which predominantly occur at the edges of an object. The authors explore this effect in the context of stem cell imaging to identify whether these changes are associated with standard forces from cell-matrix adhesions or with specific aggregations of proteins at the cell membrane level. Such aggregations are known as focal adhesions (FAs), and they regulate the engagement of the cells with their environment as well as their motility (Fig. 1). Standard cell-matrix adhesion shows a significant variation in the refractive index but has a low impact on scattering. In contrast, FAs exhibit notable variations in the refractive index and a strong increase in scattering. Therefore, the PROM technique is able to identify the

Multiparameter resonant imaging for studying cell interactions