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T T • Phenotypic cell differentiation • Physiological relevance • Patient-derived models • Stem cell models • Animal model substitute • Biological heterogeneity • Target validation • Morphological heterogeneity • Size and shape-based viability • Spatial dependence of physiological state • Simplified organ models • Validation uncertain • No vascularization system • Dynamic tuning of tumour ecosystem • Long, complicated cell culture protocols

Concerns, challenges and promises of high-content analysis of 3D cellular models