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Fungal hydrolytic enzymes have a great potential due to the rapid development of enzyme technology and their industrial applications. A fluorimetric microplate assay has been developed for measuring activity of &#x3b2;-D-exoglucanase, &#x3b2;-D-glucosidase, &#x3b1;-D-glucosidase, &#x3b2;-N-acetyl-hexosaminidase, &#x3b2;-D-xylosidase  in diluted culture broth samples. The substrates used are conjugates of the highly fluorescent compounds 4-methylumbelliferone (MUB) and thus product formation can be measured directly in the microplate without previous extraction and purification of the product. The developed system was evaluated on eight fungal strains isolated from shrub species (Tamarix) that can be used in phytoremediation. Depending upon the enzyme, each species exhibited different levels of enzymatic activities as well as different production profiles. The fluorimetric method could constitute an effective alternative to the pectrophotometric method to screen hydrolase-producing microbial strains

Rapid bioassay for evaluating enzyme production in fungal isolates from environmental sources