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Here we present a microRNA (miRNA) knockdown application, based on the use of Locked Nucleic Acid (LNA™)-modified probes. The use of LNA for the knockdown of miRNA is particularly effective because these probes have high affinity for their short RNA targets and excellent mismatch-discrimination capability. Here we demonstrate that LNA-modified probes, termed miRCURY™ LNA Knockdown probes, are highly specific and potent tools for functional analysis of miRNAs.

Functional studies of microRNA based on knockdown using Locked Nucleic Acid probes