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Existing library preparation methods for deep sequencing of mRNA (mRNA-seq) are time-consuming, multistep processes that are dependent on intact (nondegraded) total RNA samples, efficient removal of ribosomal RNA (rRNA), ligation of platform-specific adaptors, and multiple clean-up steps to generate di-tagged cDNA. We describe novel methods for rRNA removal from intact and fragmented RNA (Ribo-Zero™ technology) and for rapid preparation of directional, di-tagged mRNA-seq libraries (ScriptSeq™ technology), which together address the shortcomings of existing mRNA-seq library preparation workflows.

Novel methods for rRNA removal and directional, ligation-free RNA-seq library preparation