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To the Editor: Structural variants constitute the largest portion of nucleotide variation in genomes, yet their comprehensive characterization based on high-throughput sequencing technologies is still challenging. Here we present primer-initiated sequence synthesis for genomes (PrInSeS-G), a software tool that detects and assembles sequence variants (1 base pair (bp) to |[sim]|10 kilobases (kb)) from single- or paired-end short reads.

Primer-initiated sequence synthesis to detect and assemble structural variants