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Light sheet microscopy promises to revolutionize developmental biology byenabling live in toto imaging of entire embryos with minimal phototoxicity. Wepresent detailed instructions for building a compact and customizable SelectivePlane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardwareand software platform is shared with the scientific community through a publicwebsite, thereby making light sheet microscopy accessible for widespread useand optimization to various applications.

OpenSPIM - an open access platform for light sheet microscopy