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In vivo real-time epifluorescence imaging of mouse hindlimb vasculatures inthe second near-infrared region (NIR-II, 1.1~1.4 microns) is performed usingsingle-walled carbon nanotubes (SWNTs) as fluorophores. Both high spatialresolution (~30 microns) and temporal resolution (<200 ms/frame) for smallvessel imaging are achieved 1-3 mm deep in the tissue owing to the beneficialNIR-II optical window that affords deep anatomical penetration and lowscattering. This spatial resolution is unattainable by traditional NIR imaging(NIR-I, 0.75~0.9 microns) or microscopic computed tomography (micro-CT), whilethe temporal resolution far exceeds scanning microscopic imaging techniques.Arterial and venous vessels are unambiguously differentiated using a dynamiccontrast-enhanced NIR-II imaging technique based on their distincthemodynamics. Further, the deep tissue penetration, high spatial and temporalresolution of NIR-II imaging allow for precise quantifications of bloodvelocity in both normal and ischemic femoral arteries, which are beyond thecapability of ultrasonography at lower blood velocity.

Multifunctional in vivo vascular imaging using near-infrared II fluorescence