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Male sterility is a valuable trait for plant breeding and hybrid seed production. The dominant male-sterile gene  Ms2  in common wheat has facilitated the release of hundreds of breeding lines and cultivars in China. Here, we describe the map-based cloning of the  Ms2  gene and show that  Ms2  confers male sterility in wheat, barley and  Brachypodium .  MS2  appears as an orphan gene within the Triticinae and expression of  Ms2  in anthers is associated with insertion of a retroelement into the promoter. The cloning of  Ms2  has substantial potential to assemble practical pipelines for recurrent selection and hybrid seed production in wheat.

Wheat Ms2 encodes for an orphan protein that confers male sterility in grass species