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To the Editor Auxotrophic markers—mutations in genes encoding enzymes in pathways for the biosynthesis of metabolic building blocks, such as amino acids or nucleotides—are used as selection markers in most yeast genetics and genomics experiments1, 2, 3. The nutritional deficiency caused by the mutation (auxotrophy) can be compensated by supplying the… Download references

A prototrophic deletion mutant collection for yeast metabolomics and systems biology