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The crustacean isopod species Asellus aquaticus does not usually have recognizable sex\udchromosomes. We previously identified a Y chromosome marked by two heterochromatic bands in\udsome males of a population from the Sarno river near Naples. In this work we used oligonucleotide\udprobes to test the presence and possible sex-specific distribution of five simple repeat motifs in the\udgenome of male and female individuals from the Sarno population. The five oligonucleotide probes\udwere hybridized to enzyme-restricted genomic DNAs and the chromosome location of two probes\udwas tested using fluorescence in situ hybridization. Our results show that only the (TCC) repetitive\udsimple sequence has a sex-specific hybridization pattern and presents a significant accumulation on\udthe Y chromosome in the region included between the two heterochromatic areas. Moreover the\udGGAAT sequence is not present in the genome of A. aquaticus in any detectable quantity

Simple DNA repeats and sex chromosome differentiation in Asellus aquaticus (Crustacea, Isopoda)