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A line of human leukaemia-derived cells is described that kills a wide range of human and animal cell lines, whether normal or malignant, even at a ratio of 1:1. During exposure to the target cells, the killer cells released a factor into the culture medium which destroyed target cells in the absence of the killer cells. This phenomenon occurs without exogenous complement and requires no pre-treatment of target or killer cells. The humoral factor is a protein precipitable by 60% saturation of ammonium sulphate and has a mol. wt. of approximately 70,000; It prevented the growth of a fibrosarcoma in mice.

A human haemic cell line capable of cellular and humoral killing of normal and malignant cells