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Conversion of 7alpha3H-testosterone to 17beta-oestradiol by human mammary carcinoma tissue in vitro has been demonstrated. It was characterized unequivocally by conversion to 17beta-oestradiol-3-sulphate upon incubation with adenosine-3'-phosphate-5'-phosphosulphate and the highly specific enzyme oestrogen sulphotransferase.

Biosynthesis of 17β-oestradiol in human breast carcinoma tissue and a novel method for its characterization