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DNA MEDIATED genetic transformation is fairly established in many microbial systems including Pneumococcus (McCarty, Taylor and Avery, 1946), B. subtilis (Spizizen, 1958), E. coli. (Boivin, Vendrely and Lehoult, 1945), Haemophilus (Alexander and Leidy, 1951), etc. But in mammalian systems the problem has not been adequately explored. Results on transformation studies in intact animals are generally negative (Bearn, 1959; Kok, 1959; Shoffner et al., 1961; Svoboda and Haskova, 1959; Tigyi, Benedeczky and Lissak, 1959). With ducks however some positive evidence has been recorded (Benoit et al., 1957; Novikov, Chepinoga and Lyubarskoya, 1961). Genetic transformation has also been claimed in case of mammalian cells cultured both in vitro (Bradley et al., 1962; Kraus, 1961; Szybalska and Szybalski, 1962) and in vivo (Podgejetskaja, 1964), although there are some cases of ambiguity in the interpretation of results on transformation (Bradley, Roosa and Law, 1962). This ambiguity or failure has so far been variously accounted for by the presence of insufficient number of recipient cells (Florsheim, 1962), high frequency of spontaneous mutation (Mathis and Fisher, 1962), the non-specific protective action of DNA (Bradley et al., 1962) and lack of penetration of exogenous DNA (Yoon, 1964; Yoon and Sabo, 1964). That there are even cases in which the reported results could not be confirmed further complicate interpretation of data on mammalian transformation (Szybalska and Szybalski, 1962). In the present work a study has been made on DNA mediated genetic transformation of a clonal cell line of human uvular carcinoma isolated in this laboratory with reference to 6-azathymine (purchased from M/s. Sigma Chemical Company, U.S.A.) resistance marker.

DNA mediated genetic transformation of a human cancerous cell line cultured in vitro