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BIURKITT'S lymphoma is a malignanit neoplasm of the lymphoid tissues, which occurs most frequently amongst children in certain parts of Africa (Burkitt, 1963). Dr. Epstein and his colleagues at the Middlesex Hospital, London, have successfully set up and maintained in tissue culture, lymphoblasts obtained from tissue biopsies from three children with Burkitt's lymphoma (Epstein, Barr and Achong. 1965a, b; Epstein and Barr, 1965). Similar cultures have been grown by Stewart and her co-workers (Stewart. A virus has been demonstrated to be present in a proportion of the cells in each of the three lines grown 1965b) and in that grown by Stewart (Stewart et al., 1965). The virus, which morphologically resembles herpes. has not as yet proved identifiable but at present there is no evidence that it is an oncogenic agent. Cells from Dr. Epstein's stock have been established in several different research centres and there is now a considerable interest in these cells, not only from the point of view of the virological problems. They may also provide a convenient system on which to investigate the mode of action of anti-mitotic drugs. Detailed analysis of the mode of action of those drugs which affect DNA synthesis is greatly facilitated if information is available on the chromosome content of the cells and their proliferation kinetics. Chromosome studies were reported for EB2 one year ago: Stewart et al. (1965) showed this line to be predominantly diploid (98%0). Since then this cell-line has undergone a marked alteration to heteroploid. In this paper we report the results of an investigation of the cytogenetics of EBI, EB2 and EB3. and observations on the kinetics of their proliferation. IMETHODS AND MATERIALS The three tissue culture lines of Burkitt lymphoma cells, EBI, EB2 and EB3. were kindly supplied by Dr. Epstein. Their earlier histories of isolation and cultivation have been described previously All these lines were derived from negro children in Uganda presenting with Burkitt lymphomata. Briefly, the histories of the cells are as follows: EBI was derived from a right maxillary tumour in a 9-year-old girl. The cells were first set up in culture on December 5, 1963. EB2 came from an ovarian tumour in a 7-year-old girl and was set up in culture on May '2, 1964.

Kinetics and chromosome analyses of tissue culture lines derived from Burkitt lymphomata.