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POWELL (1961) demonstrated in healthy cultures of HeLa carcinoma cells, acute immediate damage to cells undergoing mitosis when treated with " Dextraven ", followed about two weeks after treatment, by a delayed toxicity in which whole cultures degenerated and died except for a few surviving cells. The effects of dextran solutions upon Earle's L 929 strain of mouse fibroblasts have been studied to find out if these cells showed a similar response. Preliminary experiments indicated that L cells were more resistant to treatment than were HeLa cells. Consequently, in the experiments to be described here, L cultures were treated with dextran fraction B (B.D.H.; molecular weight 150,000200,000), the most active fraction, in isotonic glucose solution. This preparation produced greater damage to HeLa cells than " Dextraven " (Powell, unpublished observations) in which the vehicle is physiological sodium chloride solution. Dextran fractions A and C (B.D.H.) appeared to be less toxic than fraction B to HeLa cells.

The Effects of Dextran on Earle's L 929 Strain of Mouse Fibrocytes