English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

FOLLOWING the demonstration by Miller and Miller (1947) of the binding of carcinogenic aminoazo dyes to proteins of the target organ, much work has been directed towards elucidating the reactive sites of both carcinogen and protein. Miller and Miller (1953) have suggested that the linkage between dye and protein is stable to treatment with hot ethanolic KOH. Under these conditions hydrolysis of protein occurs so that the recovered polar dye should have an amino acid residue attached. To date, insufficient quantities of this polar derivative have been obtained to enable chemical identification of this residue. Gelboin, Miller and Miller (1958) have reported that, a few hours after the intraperitoneal administration of a large dose of 3'-methyl-4-dimethylaminoazobenzene (3'MeDAB), protein-bound derivatives are detectable in the liver. The electrophoretic and spectral properties of the bound dyes obtained, either by prolonged feeding or following a single dose, were similar. The findings of Gelboin, Miller and Miller (1958) support the view that a large percentage of the bound dye is bound to certain liver proteins during their synthesis rather than by becoming attached to protein already formed. This interpretation is in disagreement with that of Hultin (1956) who suggested that the formation of protein bound dye is independent of protein synthesis. To date, little evidence is available concerning the actual site(s) involved in the protein(s) concerned. Kusama and Terayama (1957), Terayama and Kusama (1957), Kusama, Terayama and Teruya (1958) and Terayama, Kusama, Teruya, Kuroda and Nakayama (1958), as a result of spectroscopic studies of synthetic polar-like dyes, have suggested that a tyrosine-aminoazobenzene combination is the most probable model for the polar dye. In this communication the results ofan investigation, using isotopically labelled amino acids in an endeavour to determine the origin of substituents in the polar derivative(s) of aminoazo dyes, are presented.

Concerning the Origin of Substituents in Polar-Bound Derivatives of 3′-Methyl-4-Dimethylaminoazobenzene