English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Friend murine erythroleukaemia (F-MEL) cells are a permanent line of primitive erythroid precursors originally derived from the spleens of mice infected with the Friend strain of murine leukaemia virus. F-MEL cells differentiate in vitro in response to various chemical inducers. Concomitantly with induction, a biphasic regulation of c-myc oncogene transcripts is observed. Within one hour of the addition of dimethyl sulphoxide (DMSO) or hypoxanthine (Hyp), the levels of c-myc transcripts fall dramatically and remain virtually undetectable for the next few hours. Between 8 and 24 hours after induction, c-myc transcripts return to pre-induction levels and then decline again between 3 and 5 days as most of the cells undergo terminal differentiation. To explore the potential relationship between c-myc expression and F-MEL terminal differentiation, we have investigated here whether reversing the early fluctuations in c-myc transcript levels affects the ability of F-MEL cells to differentiate. We therefore constructed an amplifiable plasmid vector containing a full-length mouse c-myc complementary DNA and introduced it stably into recipient F-MEL cells. The exogenous c-myc sequences are transcribed in F-MEL cells and the transcript levels do not change significantly in response to inducing agents. The net result is continued c-myc expression following DMSO or Hyp induction and a complete or partial inhibition of F-MEL differentiation.

Deregulated expression of c-myc by murine erythroleukaemia cells prevents differentiation