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Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.

journal of proteome research

Rapid Isolation and Identification of Bacteriophage T4-Encoded Modifications ofEscherichia coliRNA Polymerase: A Generic Method to Study Bacteriophage/Host Interactions