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A strategy for the solid phase peptide synthesis (SPPS) and coupling of N-peptidyl and N-glycopeptidyl 2,4-dinitrobenzenesulfonamides (dNBS) with C-terminal peptidyl thioacids has been developed. The resulting N-dDNBS peptides were coupled to generate longer peptides. Ligation reactions were complete within 15 to 20 min.

Mixed-Phase Synthesis of Glycopeptides Using a N-Peptidyl-2,4-dinitrobenzenesulfonamide–Thioacid Ligation Strategy