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Eleven new withanolides (1-11) were isolated and characterized from the aerial parts of Nicandra john-tyleriana. Five of these withanolides have an unmodified skeleton (1-5), two are acnistins (6, 7), and four are withajardins (8-11). These new isolates were fully characterized using a combination of spectroscopic techniques (including multidimensional NMR) and mass spectrometry. All compounds were evaluated for their antibacterial activity against Bacillus, Enterococcus, Escherichia, Listeria, Pseudomonas, and Staphylococcus strains.

Withanolides with Antibacterial Activity from Nicandra john-tyleriana