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We recently developed a 2D 1H-13C HSQC0 approach to quantify individual chemicals in complex mixtures. The HSQC0 approach has been implemented in phase-cycled and gradient-selective versions. As in quantitative 1D NMR, the normalized integrated signal intensities in HSQC0 are proportional to the concentrations of individual chemicals in the mixture. We applied the HSQC0 approach to selectively quantify thiocoraline present at a level of 1% w/w in an extract from a Verrucosispora sp. isolated from the sponge Chondrilla caribensis f. caribensis. We expect that this approach can be used to quantify other natural products of interest in extracts without prior purification.

Selective Quantification by 2D HSQC0 Spectroscopy of Thiocoraline in an Extract from a Sponge-Derived Verrucosispora sp.

Selective Quantification by 2D HSQC₀ Spectroscopy of Thiocoraline in an Extract from a Sponge-Derived Verrucosispora sp.