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We report the first study of a network of connected enzyme-catalyzedreactions, with added chemical and enzymatic processes that incorporate therecently developed biochemical filtering steps into the functioning of thisbiocatalytic cascade. New theoretical expressions are derived to allow simple,few-parameter modeling of network components concatenated in such cascades,both with and without filtering. The derived expressions are tested againstexperimental data obtained for the realized network's responses, measuredoptically, to variations of its input chemicals' concentrations with andwithout filtering processes. We also describe how the present modeling approachcaptures and explains several observations and features identified in earlierstudies of enzymatic processes when they were considered as potential networkcomponents for multi-step information/signal processing systems.

Networked Enzymatic Logic Gates with Filtering: New Theoretical Modeling Expressions and Their Experimental Application