English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The biosynthetic crossover of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2) enzymatic activities is a productive pathway to convert arachidonic acid into unique eicosanoids. Here, we show that COX-2 catalysis with 5-LOX derived 5-hydroxy-eicosatetraenoic acid yields the endoperoxide 5-hydroxy-PGH 2 that spontaneously rearranges to 5-OH-PGE 2 and 5-OH-PGD 2 , the 5-hydroxy analogs of arachidonic acid derived PGE 2 and PGD 2 . The endoperoxide was identified via its predicted degradation product, 5,12-dihydroxy-heptadecatri-6 E ,8 E ,10 E -enoic acid, and by SnCl 2 -mediated reduction to 5-OH-PGF 2α . Both 5-OH-PGE 2 and 5-OH-PGD 2 were unstable and degraded rapidly upon treatment with weak base. This instability hampered detection in biologic samples which was overcome by in situ reduction using NaBH 4 to yield the corresponding stable 5-OH-PGF 2 diastereomers and enabled detection of 5-OH-PGF 2α in activated primary human leukocytes. 5-OH-PGE 2 and 5-OH-PGD 2 were unable to activate EP and DP prostanoid receptors, suggesting their bioactivity is distinct from PGE 2 and PGD 2 .

Biosynthetic Crossover of 5-Lipoxygenase and Cyclooxygenase-2 Yields 5-Hydroxy-PGE2 and 5-Hydroxy-PGD2

Biosynthetic Crossover of 5-Lipoxygenase and Cyclooxygenase-2 Yields 5-Hydroxy-PGE₂ and 5-Hydroxy-PGD₂