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In this paper, we describe an optimized reconstitution of the thiamin thiazole synthase (ThiG) catalyzed reaction and demonstrate that the enzymatic product is an unanticipated dearomatized thiazole tautomer.

Biosynthesis of the Thiamin Thiazole in Bacillus subtilis: Identification of the Product of the Thiazole Synthase-Catalyzed Reaction