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The Sf20-400 and the high-density lipoproteins of human sera have been degraded by partially extracting their lipid content with ethyl ether. The resultant degradation products have been analyzed both chemically and ultracentrifugally. In the case of the Sf20-400 class lipoproteins the ether extracted more than 60 o/o of the total lipids. The composition of the extracted lipids (mostly glycerides) was approximately constant for each of four successive extractions. On the other hand, the main lipid constituent of the lipoprotein fragments was phospholipid. In the high-density lipoproteins, the ether extracted only a very small amount of lipids. In spite 6Lthis resistance to ether degradation an essentially lipid-free protein fragm:eb:t was produced, the molecular weight of which was calculated to be approximately 40, 000.

Degradation of Sf20-400 and High Density Lipoproteins of Human Sera by Ethyl Ether1

Degradation of S_f20-400 and High Density Lipoproteins of Human Sera by Ethyl Ether¹