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A static light scattering (SLS) study of bovine serum albumin (BSA) mixtureswith two anionic graft copolymers of poly (sodium acrylate-co-sodium2-acrylamido-2-methyl-1-propanesulphonate)-graft-poly (N,N-dimethylacrylamide), with a high composition in poly (N,N-dimethylacrylamide) (PDMAM) side chains, revealed the formation of oppositelycharged complexes, at pH lower than 4.9, the isoelectric point of BSA. Thecore-corona nanoparticles formed at pH = 3.00, were characterized. Theirmolecular weight and radius of gyration were determined by SLS, while theirhydrodynamic radius was determined by dynamic light scattering. Small angleneutron scattering measurements were used to determine the radius of theinsoluble complexes, comprising the core of the particles. The values obtainedindicated that their size and aggregation number of the nanoparticles, weresmaller when the content of the graft copolymers in neutral PDMAM side chainswas higher. Such particles should be interesting drug delivery candidates, ifthe gastrointestinal tract was to be used.

Characterization of the Soluble Nanoparticles Formed through Coulombic Interaction of Bovine Serum Albumin with Anionic Graft Copolymers at Low pH