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A simple and green colorimetric sensing assay strategy for highly efficient determination of melamine has been fabricated, which is based on the redox reaction of gallic acid with Ag + . Monodispersed Ag nanoparticles (AgNPs) were obtained using gallic acid as a reducing and stabilizing agent. However, the aggregate behavior of AgNPs was observed, while the melamine was present in the reaction medium. As a result, the color of the solution changed from vivid yellow to brown, and the density of the color was quantitatively correlated with the melamine concentration. The aggregation of AgNPs could be attributable to the formation of hydrogen bonds between melamine and gallic acid. The designed sensor exhibited a good detection limit of 0.099 μM (0.012 ppm), which was much lower than the safety limit in China (1.0 ppm) and EU (2.0 ppm). Additionally, the sensing assay displayed good selectivity toward melamine over other coexisting substances. Consequently, the proposed colorimetric sensor was successfully used for the determination of melamine detection in raw milk samples.

In Situ Functionalization of Silver Nanoparticles by Gallic Acid as a Colorimetric Sensor for Simple Sensitive Determination of Melamine in Milk