Open AccessMagnetic Resonance Imaging of Protease-Mediated Lung Tissue Inflammation and Injury
Author(s) -
Angélique Rivot,
Natacha Jugniot,
Samuel Jacoutot,
Nicolas Vanthuyne,
Philippe Massot,
Philippe Mellet,
Sylvain R. A. Marque,
Gérard Audran,
Pierre Voisin,
Marie Delles,
Gilles Devouassoux,
Eric Thiaudière,
Abderrazzak Bentaher,
Elodie Parzy
Publication year - 2021
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.1c01150
Subject(s) - in vivo , inflammation , proteases , magnetic resonance imaging , proteolysis , lipopolysaccharide , lung , elastase , pathology , neutrophil elastase , protease , chemistry , medicine , immunology , biology , biochemistry , enzyme , radiology , microbiology and biotechnology
Pulmonary inflammation usually involves strong neutrophil recruitment with a marked release of proteases such as neutrophil elastase (NE). Noninvasive in vivo assessment of unregulated elastase activity in the lungs would provide a valuable diagnostic tool. Here, it is proposed to use Overhauser-enhanced magnetic resonance imaging (OMRI) in mice where inflammation was induced by the instillation of lipopolysaccharide (LPS). OMRI contrast in the lungs was generated by a dedicated NE free radical substrate. The free radical decayed more rapidly in LPS-treated mouse lungs than in control mice, indicating the occurrence of increased proteolysis under inflammation. Preclinical detection of abnormal proteolysis opens the way for new diagnosis modality and antiprotease testing in vivo.