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Monitoring chemical reactions in solutions at the scale of individualentities is challenging: single particle detection requires small confocalvolumes which are hardly compatible with Brownian motion, particularly whenlong integration times are necessary. Here, we propose a real-time (10 Hz)holography-based nm-precision 3D tracking of single moving nanoparticles. Usingthis localization, the confocal collection volume is dynamically adjusted tofollow the moving nanoparticle and allow continuous spectroscopic monitoring.This concept is applied to the study galvanic exchange in freely-movingcollo{\"i}dal silver nanoparticles with gold ions generated in-situ. While theBrownian trajectory reveals particle size, spectral shifts dynamically revealcomposition changes and transformation kinetics at the single object level,pointing at different transformation kinetics for free and tethered particles.

3D Spectroscopic Tracking of Individual Brownian Nanoparticles during Galvanic Exchange