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Anti-Brownian traps confine single particles in free solution by closed-loopfeedback forces that directly counteract Brownian motion. The extended-durationmeasurement of trapped objects allows detailed characterization ofphotophysical and transport properties, as well as observation of infrequent orrare dynamics. However, this approach has been generally limited to particlesthat can be tracked by fluorescent emission. Here we present theInterferometric Scattering Anti-Brownian ELectrokinetic trap (ISABEL trap),which uses interferometric scattering rather than fluorescence to monitorparticle position. By decoupling the ability to track (and therefore trap) aparticle from collection of its spectroscopic data, the ISABEL trap enablesconfinement and extended study of single particles that do not fluoresce, thatonly weakly fluoresce, or which exhibit intermittent fluorescence orphotobleaching. This new technique significantly expands the range of nanoscaleobjects that may be investigated at the single-particle level in free solution.

Interferometric scattering enables fluorescence-free electrokinetic trapping of single nanoparticles in free solution