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Ultraperformance liquid chromatography tandem mass spectrometry and Quick, Easy, Cheap, Effective, Rugged, and Safe based analytical methodologies to quantitate both free (alternariol (1), alternariol monomethyl ether (2), tenuazonic acid (3), tentoxin (4), altenuene (5), altertoxin-I (6)) and conjugated (sulfates and glucosides of 1 and 2) Alternaria toxins in fruit and vegetable juices and tomato products were developed and validated. Acceptable limits of quantitation (0.7-5.7 μg/kg), repeatability (RSDr &lt; 15.7%), reproducibility (RSDR &lt; 17.9%), and apparent recovery (87.0-110.6%) were obtained for all analytes in all matrices investigated. 129 commercial foodstuffs were analyzed, and 3 was detected in 100% of tomato product samples (&lt;LOQ to 333 μg/kg), while 1, 2, 4, and 5 were also frequently detected (21-86%, &lt;LOQ to 62 μg/kg). Moreover, low levels (&lt;LOQ to 9.9 μg/kg) of modified Alternaria toxins (sulfates of 1 and 2) were repeatedly detected. A deterministic dietary exposure assessment revealed the possible risk for human health related to the presence of 1 and 2 in tomato based foodstuffs, whereas 3 is unlikely to be of human health concern.

journal of agricultural and food chemistry

Validated UPLC-MS/MS Methods To Quantitate Free and Conjugated <i>Alternaria</i> Toxins in Commercially Available Tomato Products and Fruit and Vegetable Juices in Belgium

Validated UPLC-MS/MS Methods To Quantitate Free and Conjugated Alternaria Toxins in Commercially Available Tomato Products and Fruit and Vegetable Juices in Belgium