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Hydrothermal liquefaction of biomass produces a complex biocrude, which can be further upgraded to biofuel or chemicals, but there is a need for improved molecular understanding of product composition and reaction pathways. This study extensively characterizes semivolatile compounds in biocrudes from hydrothermal liquefaction (HTL) of microalgae (N. gaditana, C. vulgaris), macroalgae (L. hyperborea), residue (dried distillers grains with solubles), and lignocellulosic (M. × giganteus). The biocrudes were analyzed using 2D gas chromatography coupled to time-of-flight mass spectrometry with in situ silylation. A total of 73 fatty acids were detected of which C18 and C20 compounds were most diverse, while palmitic acid was the single most abundant fatty acid. Multiple fatty acid amides were detected in biocrude from N. gaditana while being almost absent in samples from other lipid- and protein-containing feedstocks. Several alkylated indoles and quinolines were observed in biocrudes from protein-containing f...

Characterizing Semivolatile Organic Compounds of Biocrude from Hydrothermal Liquefaction of Biomass