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Since  the original definition of the coli-aerogenes group of organisms by MacConkey (1905,1909), research has been continually directed in an endeavour to find a more rapid and definite distinction between its respective members.  In general, for the purposes of water analysis, the conform organisms can be divided into two main groups: faecal coli, of which  Bact. coli type I can be considered the typical member, and the non-faecal organisms comprising the intermediate-aerogenes-cloacae (I.A.C.) organisms. All available evidence tends to show that  Bact. coli type I is by far the most frequent organism in the human and animal intestine. Levine (1921) has summarized the figures of various workers and obtained a percentage ratio of 93·4:6·6 as the ratio of coli:aerogenes in faeces, while Bardsley (1938) has shown that in only a very few cases does the number of I.A.C. organisms exceed the faecal coli in faeces.

An Examination of The Modified Eijkman Method Applied To Pure Coliform Cultures Obtained From Waters in Singapore