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Papillae are minute projections which appear on colonies of bacteria growing on agar plates about the 3rd or 4th day of incubation. They occur probably on all bacterial colonies, but have been studied chiefly on members of the coli, typhoid and dysentery groups. If a plate bearing colonies of Bacillus colicommunis or B. dysenteriae Flexner is held up to the light on the 3rd or 4th day and examined with a hand lens giving x 10 magnification, the papillae can be seen by transmitted light as minute shining bright spots about 0-05 mm. in diameter. They are generally situated toward the centres of the colonies. During the next 2 days these spots increase in size to 0-25 or 0-50 mm. and definitely project above the surface. By transmitted light they are golden, by reflected light they are grey-white projections on the surface. They may grow out over the surface of the colony to its margin. On the other hand, their growth may be arrested earlier, when they measure only 0-10 mm. Papillae vary greatly in number. There may be only one or two on a number of colonies or there may be so many that they cover half the surface of any colony. III. Experiments with B. dysenteriae Flexner grown on acriflavine (Table 3) . . 338 IV. Experiments with B. dysenteriae Flexner grown on argyrol (Table 4) . . . 339 V. Experiments with B. dysenteriae Flexner grown on neosalvarsan (Table 5) . . 339 VI. The biological functions of papillae (Table 6) 339 Summary . . . . . . . 343 References . . . . . . 344

journal of hygiene

Experiments on adaptive variation and rejuvenation:<i>Bacillus dysenteriae</i>Flexner, grown on media containing sulphanilamide, acriflavine, argyrol, neosalvarsan and no drug

Experiments on adaptive variation and rejuvenation:Bacillus dysenteriaeFlexner, grown on media containing sulphanilamide, acriflavine, argyrol, neosalvarsan and no drug