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Presence of natural  Cl. welchii  α-antitoxin in 65 Indian equines was observed. All the 65 equines had 1 unit or more of circulating natural antitoxin per ml. of serum. The maximum titre observed was 4 units/ml. in about 8% of the animals. These figures are very much higher than the figures obtained in other countries. Such a high incidence of natural immunity indicates frequent natural contact with the antigen concerned.  Results of hyperimmunization of 7 equines are recorded and discussed.  Our thanks are due to the authorities of Bengal Immunity Laboratory for permission to publish these observations and to Sri M. Pal for useful technical assistance.

The incidence of natural Clostridium welchii α-antitoxin in Indian equines: its influence on the results of antigenic stimulus