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The methods recommended for diagnosis of Pasteurella pestis infection in animal tissues include microscopic examination of stained impression smears, bacteriological culture followed by biochemical and bacteriophage tests, animal inoculation, and precipitin tests using soluble plague antigens (Baltazard et al. 1956). Bacteriological culture and animal inoculation are sensitive and specific when test materials are well preserved, but these methods seldom give positive results when the specimens are from putrefied or mummified carcasses. In such cases, the modified Ascoli test developed by Larson, Philip, Cicht & Hughes (1951) or the haemagglutination and complement-fixation tests of Chen, Quan & Meyer (1952) and Chen & Meyer (1954) have proved to be of value. Larson reported significant precipitinogen titres in animal tissues stored at 37° C. for periods as long as 14 weeks. Chen reported excellent results with complement fixation tests of plague antigens extracted from decomposed carcasses and from partially desiccated tissues held at 37° C. for 10 days.

Efficacy of fluorescent antibody methods for detection ofPasteurella pestisin carcasses of albino laboratory mice stored for various periods