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PART I. HISTORICAL SURVEY PAGE Introduction .86 The colon group as faecal indicators 87 (1) The B. coli test, its uses and limitations .. .87 (2) Differentiation methods for the colon-aerogenes group .. . 91 A. Distribution of the group in nature ..91 B. The fermentation of carbohydrates and indol production. .92 C. The gas ratios, the methyl-red test and the Voges-Proskauer reaction 93 D. Utilisation of organic acids and their salts . .96 E. Growth of coliform organisms at a temperature above 370 C., and its significance 99 Fallacies in the application of the foregoing methods to the bacteriological examination of water .100 A. The fallacies encountered during initial isolation .100 B. Limitations of differentiation methods .101 C. Difficulties in interpreting results .103

journal of hygiene

The Classification of the Colon-Aerogenes Group of Bacteria in Relation to their Habitat and its Application to the Sanitary Examination of Water Supplies in the Tropics and in Temperate Climates. A Comparative Study of 2500 Cultures