English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

BEFORE the year 1925 melioidosis had not been recognised outside the towns of Rangoon, Kuala Lumpur and Singapore. We had long thought it probable that the disease has a much wider distribution and that the apparent freedom of the country districts was due to the absence of laboratory facilities and the almost insuperable difficulty of diagnosing melioidosis without recourse to the laboratory. In February 1925, a case of the disease was recognised more than a hundred miles from Kuala Lumpur, a Tamil who died on the twenty-sixth day of his illness at a village about half-way between Kuala Lumpur and Singapore; B. whitmori was cultivated from his spleen, liver and lungs which had been sent for examination to the Institute for Medical Research. Recently it has been discovered that melioidosis occurs in French IndoChina. In January 1926, Dr F. H. Guerin, Director of the Pasteur Institute of Saigon, very kindly sent us a culture of B. whitmori which had been isolated by Drs R. Pons and M. Advier from the blood of a native woman. An account of the case precedes this note; it appears to have been an example of the septicaemic form of melioidosis. When the Saigon culture was plated out on eosin-methylene-blue agar, two kinds of colonies appeared; one, the ordinary corrugated or rugose type and the other, an ultra-rugose form, very corrugated and wrinkled, and so sticky that if it were touched with a needle it adhered to it and peeled off the surface of the medium. Colonies of both kinds were inoculated into liquid media; in both the organisms were motile, but those from the ultra-rugose colonies were much more active than the others. The ultra-rugose type of organism produced a corrugated growth on glycerine agar within twenty-four hours, the ordinary type grew luxuriantly but corrugation did not appear until after forty-eight hours. Both kinds fermented lactose, saccharose, glucose, mannite, dulcite, maltose and dextrin with the production of acid but no gas. Indol was not formed by either. Cultures made from the ordinary type of colony were agglutinated to full titre by immune sera prepared with strains of B. whitmori isolated in Kuala Lumpur. Cultures made from the ultra-rugose colonies were agglutinated to 30 per cent, of full titre.

Melioidosis: Notes on a culture of B. whitmori from Saigon.