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A NUMBER of methods have been described for anaerobic cultivation in separate plates. The principle offers certain advantages over the cultivation of a number of plates in the same anaerobic container, which has to be opened whenever it is desired to inspect any particular culture; and it is useful where anaerobic plate cultures are needed only occasionally or in small numbers. A few methods are well known and have been widely used. These, however, depend upon alkaline pyrogallate for absorption of oxygen; and this principle is not always dependable, being especially liable to fail with light inoculations of pure culture of anaerobes. A method was accordingly devised for single anaerobic plate cultivation in an atmosphere of hydrogen; in this the residual oxygen is removed by means of a heated capsule of asbestos-wool impregnated with carbonised palladium, after the method of Mclntosh and Fildes. A very perfect degree of anaerobiosis is thus quickly and conveniently obtained.

An Apparatus for anaerobic Plate Cultivation in Hydrogen for separate Petri Capsules: (A Report to the Food Investigation Board)